Analysis Note

ControlNon-boiled & non-B-ME HeLa cell lysate

Application

Anti-Integrin alpha-11 Antibody is an antibody against Integrin alpha-11 for use in WB.

Research Sub CategoryIntegrins

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin alpha-11 (ITGA11) belongs to a family of cell adhesion molecules, which consist of noncovalent associated alpha-beta heterodimers, and is shown to be overexpressed in the stroma of human non-small-cell lung carcinomas (hNSCLCs). Upon dimerization, this protein is thought to enhance tumorgenicity of hNSCLCs by interacting with matrix collagen and stromal fibroblasts. Progesterone is shown to increase the expression of ITGA11 through mid-gestation and decrease thereafter in Sprague-Dawley rats. It is further indicated that MAPK signaling is associated with the regulation of these integrins.

Immunogen

Linear peptide corresponding to human Integrin alpha-11.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Integrin alpha-11 in 10 µg of non-boiled & non-B-ME HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~135 kDa observed.
The high molecular weight band seen in a HeLa (non boiled) cell lysate is the integrin complex. The band at ~75-80 kDa is thought to be a denatured form of integrin.